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流式检测全体计划(CD4+CD25+FOXP3+) Treg,调理性T细胞(Treg)

宣布者:艾美捷科技宣布时候:2021-03-09

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       调理性T细胞(regulatory T,Treg)是一类节制体内免疫反映性的T细胞亚群,按照来历的差别,Treg 细胞可分为自然性Treg (natural Treg, nTreg) 和引诱性 Treg(induced Treg, iTreg)。


调理性T细胞


       nTreg由胸腺分解而来,占外周血CD4+ T细胞的5%~10%,这类细胞自身缺少增殖才能,但具备自然免疫按捺感化,不只可按捺CD4+或CD8+ T细胞的活化和增殖,还可按捺初始T细胞和影象性T细胞的功效。iTreg首要存在于外周血和肿瘤构造中,由抗原或按捺性因子如IL-2、TGF-β安慰初始CD4+ T细胞发生,对多种免疫细胞具备按捺感化。


       调理性T(regulatoryT,Treg)细胞对调理免疫稳态和按捺差别疾病情况中的免疫反映具备相当首要的感化,它能经由过程按捺CD4+帮助性T细胞和CD8+细胞毒性T细胞的激活和分解,对自体和肿瘤抒发的抗原发生反映性。Treg细胞抒发特同性转录因子Foxp3,因此,CD4+CD25+Foxp3+被以为是Treg细胞的典范标记组合。


       虽然如斯,选甚么亚型,选甚么来历,选甚么荧光?照旧是小火伴们为CD4+CD25+Foxp3+抗体组合的挑选的一大搅扰!


       作为专业的流式抗体与处理计划,艾美捷科技,为您保举流式科研与诊断计划带领者EXBio的CD4+CD25+FOXP3+Treg调理性T细胞检测试剂盒(TregFlowEx Kit)


(CD4+CD25+FOXP3+) Treg调理性T细胞检测试剂盒


TregFlowEx-Kit.jpg

(CD4+CD25+FOXP3+) Treg调理性T细胞检测试剂盒
TregFlowEx Kit
货号:ED7417
规格:50test
反映种属
利用范例流式细胞术,FC
试剂盒道理用于流式细胞术检测人外周血或脐带血中的调理性T细胞Treg (CD4 + CD25 + FOXP3 +)的百分比。
产物申明书


      试剂盒组分:


组分称号情势规格
ED7417-1牢固与裂解液10X稀释液10ml(1瓶)
ED7417-2破膜剂即用型25ml(1瓶)
ED7417-3封锁缓冲液即用型2.5ml(1瓶)
ED7417-4CD4-FITC/CD25-PE抗体预混液即用型500ul(1瓶)
ED7417-5FOXP3-APC抗体即用型250ul(1瓶)


      (CD4+CD25+FOXP3+) Treg调理性T细胞检测试剂盒尝试流程


      1.Surface staining:


      1.1 Add 10 ?l of CD4 FITC/CD25 PE reagent to tubes.

      1.2 Add 100 ?l of human blood.

      1.3 Mix well and incubate for 10 minutes in the dark in the refrigerator (2?8℃).

      1.4 (optional wash) Wash cells by adding 2 ml of cold PBS and centrifuge at 300 g for 5 minutes at 4℃.

      1.5 Aspirate supernatant and proceed immediately to the intracellular staining.


      2.Intracellular staining


      2.1 Add 1 ml of the diluted cold Fix and Lysing Solution.

      2.2 Mix and incubate for 10 minutes in the dark in the refrigerator (2?8℃).

      2.3 Add 0.5 ml of Permeabilizing Solution.

      2.4 Mix and incubate for 10 minutes in the dark in the refrigerator (2?8℃).

      2.5 Centrifuge the cells at 400 g for 5 minutes at 4℃. (Do not add any other buffer to the Fix/Perm mixture before centrifugation)

      2.6 Remove (decant) supernatant.

      2.7 Add 50 ?L of cold Blocking Buffer.

      2.8 Add 50 ?L of cold PBS.

      2.9 Mix and incubate for 5 minutes in the dark in the refrigerator (2?8℃).

      2.10 Add 5 μl of FoxP3 APC reagent.

      2.11 Mix and incubate for 30 minutes in the dark in the refrigerator (2?8℃).

      2.12. Wash cells twice with 2 ml of cold PBS, centrifugate at 400 g for 5 minutes at 4 ℃. Aspirate/decant supernatant.

      2.13. Add 300 ?l of 1% formaldehyde in PBS and store the stained samples at 2?8℃ until measured in flow cytometer. Analyze the processed samples within 4 hours.


      (CD4+CD25+FOXP3+) Treg调理性T细胞检测试剂盒成果展现:


      利用TregFlowEx试剂盒中对人CD4阳性淋巴细胞停止流式细胞术多色染色。 Treg细胞(CD4 + CD25 + FOXP3 +)位于右上象限。


TregFlowEx试剂盒


      【颁发文章】


      Cern?, V., et al. "Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns. Biomedicines 2021, 9, 170." (2021).

      Morizawa, Yosuke, et al. "Correlation of immune cells and cytokines in the tumor microenvironment with elevated neutrophil-to-lymphocyte ratio in blood: an analysis of muscle-invasive bladder cancer."Cancer investigation7 (2018): 395-405.

      Hrd?, Ji?í, et al. "Decreased allergy incidence in children supplemented with E. coli O83: K24: H31 and its possible modes of action."European journal of immunology12 (2018): 2015-2030.

      Ond?ejíková, Leona.Rozdílné vlastnosti T regula?ních buněk v pupe?níkové krvi novorozenc? zdrav?ch a alergick?ch matek. BS thesis. ?eské vysoké u?ení technické v Praze. Vypo?etní a informa?ní centrum., 2017.


      【干货】CD家属流式抗体大全(高清大图):


CD家属流式抗体大全


      EXBIO是捷克一家闻名的生物试剂公司,专一于流式细胞术配套试剂的开辟。自1990年景立以来,EXBIO公司研发和制作的抗体分销环球。今朝,EXBIO可供给跨越4000种质优、价廉的研讨和临床利用抗体,涵盖了血液学,免疫学,癌症,干细胞和细胞生物学等范畴,如:CD份子、细胞粘附份子、细胞骨架、凋亡、细胞旌旗灯号传导、肿瘤标记物、人血型、HLA、生殖等研讨用抗体,触及荧光染料如FITC、PE、PE-Cy5、PE-Cy7、APC、PerCP、PerCP-Cy5.5、Pacific Blue、Pacific Orange、Alexa Fluor 488、Alexa Fluor 647、Alexa Fluor 700、Biotin等多达13种,知足客户各种尝试需要。


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